![]() * BN, DA and WOKW are significantly different at 1 ** or 0.1*** per cent level. Relative gene expression in bone (A), subcutaneous (B) and visceral (C) adipocytes of 30 weeks old males (mean ± SEM). P-values between strains atage 10 and 30 weeks These organs were chosen because DA and BN rats are slim and WOKW rats are obese and the bone architecture is different between DA and BN BN rats tend to have fragile vertebrae, whereas DA rats primarily tend to have fragile femoral necks. Hence, we studied the expression of 20 selected genes in two organs, adipose tissue and bone the genes selected play a role in bone and lipid metabolism as well as in immunologic reactions. Furthermore, these rat strains were also used for gene expression studies, as variation in gene expression is heritable and has been mapped to the genome in humans and model organisms. Because of this phenotypic diversity, the aim of our study was to analyzed several phenotypic traits between BN, DA, and WOKW rats at an age of 10 and 30 weeks, and additionally we studied the life expectancy. Wistar Ottawa Karlsburg RT1 u (WOKW) rats are white in color (albino), powerfully built and develop facets of metabolic syndrome with obesity, dyslipidemia, insulin resistance, hyperinsulinemia, hyperleptinemia and glucose intolerance –. ĭark agouti (DA) rats are agouti in colour, slim, and susceptible to allergic encephalomyelitis, collagen induced arthritis and other auto-immune diseases. ![]() ![]() Comparative studies showed that BN rats are obviously different in genome compared to several other inbred rat strains –. It was BN rats that were used to encode the rat genome. They are also susceptible to the development of mercury-induced autoimmunity to renal basement membranes with the development of membraneous glomerulonephritis. This prompted us to study phenotypic traits and the expression of selected genes in three inbred rat strains to show the diversity of rat strains.īrown Norway (BN) rats are slim, brown in color, have very soft fur, are susceptible to respiratory inflammation, but resistant to the induction of experimental allergic encephalomyelitis. Therefore, knowledge of phenotypic and genetic variation between strains will be useful to obtain insight into the relationship between different strains. Rat inbred strains are widely used as laboratory models in understanding basic biology and human health and disease. Comparing the gene expression in visceral and subcutaneous adipocytes, only one gene showed a comparable behavior ( Bmp1).ĭespite the recent advances in human genetics, animal models of human diseases remain attractive tools, not only to overcome genetic complexity, but also to permit studies under stable environmental conditions. There were no significant differences in gene expression of one gene in subcutaneous adipocytes and of 3 genes in visceral adipocytes. The ranking BN = DA>WOKW was observed in only one gene in subcutaneous ( Fto) and visceral adipocytes ( Col6a1). In adipose tissues, Nfkb1 is only expressed in subcutaneous adipocytes, and 5 genes, Col2a1, Mmp9, Tnfa, Ins1 and Cyp24a1, are not expressed in adipocytes. The highest gene expression was found in bone of BN rats. Regarding life expectancy, BN rats lived longest (1072☒28d). There were significant differences in body weight, serum lipids and leptin at an age of 30 weeks between strains. At an age of 30 weeks, these male rats were killed by an overdose of anesthetic (Sevofluran, Abbott), and the subcutaneous and visceral adipose tissue as well as bone tissue were removed to study the expression of 20 genes. In addition, a total of 95 rats were studied for life expectancy. ![]() We studied phenotypic traits: of each strain – BN/K, DA/K and WOKW –10 male rats were studied for body weight and serum constituents at an age of 10 and 30 weeks.
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